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KMID : 1137020100210040255
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Journal of Gynecologic Oncology
2010 Volume.21 No. 4 p.255 ~ p.261
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Apoptosis-related mRNA expression profiles of ovarian cancer cell lines following cisplatin treatment
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Yoon Joo-Hee
Kim Eung-Sam
Lee Sung-Jong
Park Chang-Wook
Cha Hyung-Jin
Hong Bee-Hak
Choi Kwan-Yong
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Abstract
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Objective: The aim of this study was to identify apoptosis-related genes of ovarian cancer cell lines following cisplatin treatment.
Methods: We used IC50 values and fluorescence-activated cell sorting analysis to compare cell death in 2 ovarian cancer cell lines, namely, SKOV-3 and OVCAR-3, upon treatment with cisplatin. Moreover, the change in transcriptional levels of apoptosis-associated genes was measured with a dendron-modified DNA microarray.
Results: The protein levels for the up-regulated genes in each cell line were validated to identify the molecules that may determine the cellular behavior of cisplatin resistance. Eight genes were over-expressed in the 2 cell lines. The cisplatin-induced up-regulation of DAD1 in transcriptional and protein levels contributed to the cisplatin resistance of OVCAR-3, and the up-regulation of FASTK and TNFRSF11A in SKOV-3 resulted in its higher sensitivity to cisplatin than that of OVCAR-3.
Conclusion: In the present study, we have identified a set of genes responsible for apoptosis following cisplatin treatment in ovarian cancer cell lines. These genes may give information about the understanding of cisplatin-induced apoptosis in ovarian cancer.
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KEYWORD
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Ovarian cancer cell lines, Cisplatin, Apoptosis, Dendron-modified DNA microarray
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